Anatabine reduces hyperactivity and disinhibition in 12.5 month-old Tg PS1/APPswe mice.

<p>12.5 month-old Tg PS1/APPswe and their control littermates tested in EPM after treatment duration of 2.5 months with anatabine. A) Representative path tracks in the closed and open arms of the elevated plus maze by 12.5 months old Tg PS1/APPswe and their control wild-type littermate receiving regular drinking water (placebo) and anatabine at a dosage of 10 and 20 mg/Kg/Day in their drinking water are shown. B and C) The histogram represents the total distance travelled and mean velocity of Tg PS1/APPswe mice and their control wild-type littermates receiving regular drinking water (placebo) and anatabine at a dosage of 10 or 20 mg/Kg/Day. ANOVA showed a significant main effect of the genotype for the distance travelled (P<0.008) and mean velocity (P<0.008). Post-hoc comparisons show that Tg PS1/APPswe placebo mice were significantly different compared to their wild-type littermate mice for distance travelled (P<0.027) and velocity (P<0.027). Tg PS1/APPswe mice receiving anatabine at a dosage of 10 or 20 mg/Kg/Day were not significantly different from wild-type mice (P>0.05). D) The histogram represents the average amount of time spent by Tg PS1/APPswe and wild-type mice in open arms of the elevated plus maze. ANOVA reveals a significant main effect of the genotype (P<0.003) and an interaction between genotype and anatabine (P<0.029) for the time spent in the open arm. Post-hoc comparison shows that Tg PS1/APPswe placebo mice spent significantly more time in the open arms than wild-type control mice (P<0.002). Tg PS1/APPswe mice receiving anatabine at a dosage of 20 mg/Kg/Day spent significantly less time in the open arms of the EPM compared to Tg PS1/APPswe receiving regular drinking water (placebo) (P<0.011). Overall, anatabine treated Tg PS1/APPswe and wild-type littermate mice spent the same amount of time in the open arms (P>0.183 and P>0.683). The number of individuals (n) tested in three groups were I) Mice receiving regular drinking water (placebo): wild-type (n = 8) and Tg PS1/APPswe (n = 8); II) Mice receiving anatabine at a dosage of 10mg/Kg/Day: wild-type (n = 10) and Tg PS1/APPswe (n = 8) and III) Mice receiving anatabine at a dosage of 20mg/Kg/Day: wild-type (n = 10) and Tg PS1/APPswe (n = 8).</p>